Associate Professor, UCSF Microbiology and Immunology Divisions of Infectious Diseases and Rheumatology, Department of Medicine, and UCSF Diabetes Center Scientific Director of Biomedicine, Innovative Genomics Institute (IGI) Chan Zuckerberg Biohub Investigator
Address
513 Parnassus Avenue HSE, #444 San Francisco, CA94143 United States
Our lab's goal is to understand the genetic circuits that control human immune cell function in health and disease. We have begun to identify how genetic risk variants for autoimmune diseases disrupt immune cell circuits (Farh and Marson et al., Nature 2015; Simeonov et al., Nature, 2017), and how pathogenic circuits may be targeted with novel therapeutics (Xiao et al., Immunity 2014). My lab has developed new tools for efficient CRISPR genome engineering in primary human T cells (Schumann et al., PNAS 2015; Roth et al., Nature 2018; Nguyen et al. Nature Biotech 2020). Now we are pursuing a comprehensive strategy to test how coding and non-coding genetic variation control essential programs in the human immune system (Simeonov et al. Nature, 2017; Shifrut et al., Cell 2018; Roth et al., Cell 2020; Cortez et al., Nature 2020; Schumann et al., Nature Immunology 2020). Genome engineered human T cells hold great potential for the next generation of cell-based therapies for cancer, autoimmunity and infectious diseases.
Education
Institution
Degree
Dept or School
End Date
Harvard Medical School
M.D.
Medicine
2010
Massachusetts Institute of Technology (MIT)
Ph.D.
Biology
2008
University of Cambridge
M.Phil
Biological Sciences
2003
Harvard College
A.B.
Biology
2001
Awards & Honors
Award
Conferred By
Date
Elected Member of Association of American Physicians (AAP)
2022
CRI Lloyd J. Old STAR Career Award
2019
Elected Member of American Society of Clinical Investigators (ASCI)
2019
Chan Zuckerberg Biohub Investigator
2017
American Society of Clinical Investigation (ASCI) Young Physician-Scientist Award
2016
Burroughs Wellcome Foundation Career Award for Medical Scientists
2016
NIDA/NIH Avenir New Innovator Award
2016
Parker Institute for Cancer Immunotherapy, Project Member
2016
Grants and Funding
Transcriptional and post-transcriptional regulation of human Treg identity and function | NIH | 2026-02-06 - 2030-01-31 | Role: Principal Investigator
Decoding and reprogramming T cells through synthetic biology for cancer immunotherapy | NIH | 2023-01-01 - 2027-12-31 | Role: Principal Investigator
Inherited T cell defects: Diagnosis, Mechanisms and Treatments | NIH | 2020-09-08 - 2025-08-31 | Role: Principal Investigator
Functional Molecular Investigation of Inflammatory Bowel Disease (IBD) Risk Variants | NIH | 2018-09-18 - 2022-05-31 | Role: Principal Investigator
Functional Interrogation of Non-Coding Type 1 Diabetes Risk Variants in Human Immune Cells and Beta Cells | NIH | 2016-09-30 - 2021-05-31 | Role: Principal Investigator
Functional Testing of Host Genes that control HIV Latency in Primary Immune Cells | NIH | 2016-06-01 - 2021-05-31 | Role: Principal Investigator
Publications
MOST RECENT PUBLICATIONS FROM A TOTAL OF 139
Harmonizing standards and resources for the medical genome.
Ashley EA, Alizadeh AA, Armitage H, Bhatt AS, Blumenfeld Y, Carroll A, Chavez RM, Giannikopoulos P, Grove ME, Halley MC, Khush K, Lennon NJ, Maragh S, Marson A, Paten B, Phillippy AM, Porteus MH, Rehm HL, Ringeisen BR, Salzman J, Schneider VA, Sedlazeck FJ, Steinmetz LM, Urnov FD, Wyman SK, Zook JM, Minor LB, Doudna JA| | PubMed
Segmentation-free analysis of live-cell imaging data reveals how T cell modifications influence cancer cell aggregation dynamics.
Epstein L, Weiner AC, Verma A, Saeidi M, Carnevale J, Marson A, Engelhardt BE| | PubMed
Host Genetic Regulation of NLRP3 Inflammasome Cytokines Reveals Immune and Vascular Pathways in HIV.
Chung R, Chalasani NS, Barbehenn AS, Lundgren E, Savur S, Shome S, Sheikhzadeh CH, Sarvadhavabhatla S, Donaire MS, Pae V, Chu X, Winder D, Maguire CT, Topal S, Ganesan A, Yabes JM, Larson DT, Lalani T, Ewers EC, Colombo RE, Dugan E, Rathore U, Marson A, Agan BK, Tomalka JA, Sekaly RP, Ioannidis NM, Lee SA| | PubMed
Development of a Fully Non-Viral 1XX-enhanced BCMA CAR-T Cell Therapy for Multiple Myeloma.
Talbot A, Li K, Lee JHJ, Lang S, Liu C, Kalter N, Li Z, Mortazavi Y, Almudhfar N, Muldoon JJ, Allain V, Nyberg W, Chung JJ, Wang C, Qi Z, Krishnappa N, Ha AS, Kong D, Houser D, Paruthiyil S, Ahmadi M, Ji Y, Rosenberg M, Acevedo LA, Liang B, Briseno K, Kwek SS, Bachl S, Ching C, Carnevale J, Giannikopoulos P, Oh DY, Marson A, Hendel A, Martin T, Eyquem J, Shy BR| | PubMed
Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cells.
Rathore U, Dugan E, Thornton H, Kumar VE, Dajani R, Burdick RC, Young JM, Steinhart Z, Lao R, Delviks-Frankenberry KA, Choi W, Henriques WS, Echeverria I, Dann E, Dureja I, Pathak N, Arce MM, McKetney J, Umhoefer JM, Parulekar S, Schmidt R, Polacco BJ, Neidleman J, Montano M, Nguyen VQ, Sali A, Levy JA, Tenthorey JL, Cheng Y, Roan NR, Swaney DL, Kaake RM, Dodgson SE, Hiatt J, Pathak VK, Malik HS, Krogan NJ, Marson A| | PubMed
Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells.
Jung H, Devant P, Ching C, Ota M, Hamilton J, Steinhart Z, Ngo W, Sandoval L, Jung JH, Xu D, An M, Urs E, Chen PA, Allain V, Tada T, Nuñez JK, Landau NR, Liu DR, Eyquem J, Doudna JA, Marson A, Carnevale J| | PubMed
Causal modelling of gene effects from regulators to programs to traits.
Ota M, Spence JP, Zeng T, Dann E, Milind N, Marson A, Pritchard JK| | PubMed
Gene-corrected regulatory T cell therapy for IL2RA deficiency.
Ha AS, Kalter N, Rosenberg M, Acevedo LA, Liang B, Liu W, Paruthiyil S, Sinha M, Vu A, Nguyen V, Qi Z, Krishnappa N, Shu J, Yu J, Catanzaro J, Bluestone JA, Tang Q, Urnov FD, Marson A, Hendel A, Herold KC, Shy BR, Esensten JH| | PubMed
FOXP3 expression depends on cell-type-specific cis-regulatory elements and transcription factor circuitry.
Umhoefer JM, Arce MM, Kasinathan S, Whalen S, Dajani R, Subramanya S, Goudy L, Belk JA, Zhou R, Pham MTN, Zhang W, Hernandez R, Tran C, Kirthivasan N, Freimer JW, Mowery CT, Nguyen V, Ota M, Gowen BG, Simeonov DR, Curie GL, Li Z, Chen AY, Corn JE, Chang HY, Tang Q, Gilbert LA, Satpathy AT, Pollard KS, Marson A| | PubMed
Site-specific DNA insertion into the human genome with engineered recombinases.
Integrated epigenetic and genetic programming of primary human T cells.
Goudy L, Ha A, Borah AA, Umhoefer JM, Chow L, Tran C, Winters A, Talbot A, Hernandez R, Li Z, Subramanya S, Arab A, Kale N, Lee JHJ, Muldoon JJ, Liu C, Schmidt R, Santangelo P, Carnevale J, Eyquem J, Shy BR, Marson A, Gilbert LA| | PubMed
Base-editing a single missense mutation in A20 enhances CAR-T cell efficacy.
Blaisdell A, Bachl S, Sandoval LR, Ching C, Bowman CJ, Kale N, Prabandham M, Diolaiti M, Havig C, Advincula R, Lenci N, Li Z, Yamashita E, Wang CH, Zhang S, Liu Q, Achacoso P, Stibor D, Øynebråten I, Seo J, Ashworth A, Marson A, Ye CJ, Malynn BA, Eyquem J, Carnevale J, Ma A| | PubMed
Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function.
Allain V, Rothrock AG, Bernard PL, Nyberg WA, Talbot A, Muldoon JJ, Chung JY, To A, Chang CR, Kimmerly GR, Liu C, Tsao T, Mortazavi Y, Seo J, Li Z, Shemesh A, Schmidt R, Ward CC, Marson A, Lanier LL, Aguilar OA, Eyquem J| | PubMed
In vivo genome-wide CRISPR screens in human T cells to enhance T cell therapy for solid tumors.
Liu Q, Chen PA, Urs E, Zhang S, Arce MM, Wang CH, Li Z, Seo J, Kale N, LaFlam TN, Peng F, Shifrut E, Allen GM, Eyquem J, Fuh K, Dodgson SE, Cyster JG, Marson A, Carnevale J| | PubMed
NKp30 and NKG2D contribute to natural killer cell-mediated recognition of HIV-infected cells.
Pi R, Zhao NQ, Bien AJ, Ranganath T, Seiler C, Holmes S, Marson A, Nguyen DN, Blish CA| | PubMed
Multiplexed epigenetic memory editing using CRISPRoff sensitizes glioblastoma to chemotherapy.
Lin K, Zou C, Hubbard A, Sengelmann S, Goudy L, Wang IC, Sharma R, Pak J, Foster K, Ozawa T, de Groot JF, Phillips J, Vasudevan HN, Raleigh DR, Marson A, Murthy N, Gilbert LA, Berger MS, Liu SJ| | PubMed
Intentional heterogeneity in autologous cell-based gene therapies: strategic considerations for first-in-human trials.
Cabanski CR, Yang E, Stewart MD, Allen JD, Connolly JE, Dugan U, Greenberg PD, Mackall CL, June CH, Marson A, Maus MV, Ribas A| | PubMed
Mapping the extracellular molecular architecture of the pAg-signaling complex with α-Butyrophilin antibodies.
Ramesh A, Roy S, Slezak T, Fuller J, Graves H, Mamedov MR, Marson A, Kossiakoff AA, Adams EJ| | PubMed
SEED-Selection enables high-efficiency enrichment of primary T cells edited at multiple loci.
Chang CR, Vykunta VS, Lee JHJ, Li K, Kochendoerfer C, Muldoon JJ, Wang CH, Mazumder T, Sun Y, Goodman DB, Nyberg WA, Liu C, Allain V, Rothrock A, Ye CJ, Marson A, Shy BR, Eyquem J| | PubMed
Causal modeling of gene effects from regulators to programs to traits: integration of genetic associations and Perturb-seq.
Ota M, Spence JP, Zeng T, Dann E, Marson A, Pritchard JK| | PubMed
Cis-Regulatory Element and Transcription Factor Circuitry Required for Cell-Type Specific Expression of FOXP3.
Umhoefer JM, Arce MM, Whalen S, Dajani R, Goudy L, Kasinathan S, Belk JA, Zhang W, Zhou R, Subramanya S, Hernandez R, Tran C, Kirthivasan N, Freimer JW, Mowery CT, Nguyen V, Ota M, Gowen BG, Simeonov DR, Curie GL, Li Z, Corn JE, Chang HY, Gilbert LA, Satpathy AT, Pollard KS, Marson A| | PubMed
Central control of dynamic gene circuits governs T cell rest and activation.
Arce MM, Umhoefer JM, Arang N, Kasinathan S, Freimer JW, Steinhart Z, Shen H, Pham MTN, Ota M, Wadhera A, Dajani R, Dorovskyi D, Chen YY, Liu Q, Zhou Y, Swaney DL, Obernier K, Shy BR, Carnevale J, Satpathy AT, Krogan NJ, Pritchard JK, Marson A| | PubMed
Antigen-Specific T Cell Receptor Discovery for Treating Progressive Multifocal Leukoencephalopathy.
Gupta S, Martinov T, Thelen A, Sunahara M, Mureli S, Vazquez A, Gerdts J, Dandekar R, Cortese I, Fouassier C, Schanzer E, Urnov FD, Marson A, Shy BR, Greenberg PD, Wilson MR| | PubMed
Method of moments framework for differential expression analysis of single-cell RNA sequencing data.
Kim MC, Gate R, Lee DS, Tolopko A, Lu A, Gordon E, Shifrut E, Garcia-Nieto PE, Marson A, Ntranos V, Ye CJ| | PubMed
Gene regulatory network inference from CRISPR perturbations in primary CD4+ T cells elucidates the genomic basis of immune disease.
Weinstock JS, Arce MM, Freimer JW, Ota M, Marson A, Battle A, Pritchard JK| | PubMed
Phenotypic profiling of human induced regulatory T cells at early differentiation: insights into distinct immunosuppressive potential.
Kattelus R, Starskaia I, Lindén M, Batkulwar K, Pietilä S, Moulder R, Marson A, Rasool O, Suomi T, Elo LL, Lahesmaa R, Buchacher T| | PubMed
NKp30 and NKG2D contribute to natural killer recognition of HIV-infected cells.
Zhao NQ, Pi R, Nguyen DN, Ranganath T, Seiler C, Holmes S, Marson A, Blish CA| | PubMed
A model for accurate quantification of CRISPR effects in pooled FACS screens.
Pimentel H, Freimer JW, Arce MM, Garrido CM, Marson A, Pritchard JK| | PubMed
Systematic decoding of cis gene regulation defines context-dependent control of the multi-gene costimulatory receptor locus in human T cells.
Mowery CT, Freimer JW, Chen Z, Casaní-Galdón S, Umhoefer JM, Arce MM, Gjoni K, Daniel B, Sandor K, Gowen BG, Nguyen V, Simeonov DR, Garrido CM, Curie GL, Schmidt R, Steinhart Z, Satpathy AT, Pollard KS, Corn JE, Bernstein BE, Ye CJ, Marson A| | PubMed
Long noncoding RNA LIRIL2R modulates FOXP3 levels and suppressive function of human CD4+ regulatory T cells by regulating IL2RA.
Andrabi SBA, Kalim UU, Palani S, Khan MM, Khan MH, Fagersund J, Orpana J, Paulin N, Batkulwar K, Junttila S, Buchacher T, Grönroos T, Toikka L, Ammunet T, Sen P, Orešic M, Kumpulainen V, Tuomisto JEE, Sinha R, Marson A, Rasool O, Elo LL, Lahesmaa R| | PubMed
A proximal enhancer regulates RORA expression during early human Th17 cell differentiation.
Kalim UU, Biradar R, Junttila S, Khan MM, Tripathi S, Khan MH, Smolander J, Kanduri K, Envall T, Laiho A, Marson A, Rasool O, Elo LL, Lahesmaa R| | PubMed
Bidirectional epigenetic editing reveals hierarchies in gene regulation.
Pacalin NM, Steinhart Z, Shi Q, Belk JA, Dorovskyi D, Kraft K, Parker KR, Shy BR, Marson A, Chang HY| | PubMed
Publisher Correction: Advancing T cell-based cancer therapy with single-cell technologies.
Bucktrout SL, Banovich NE, Butterfield LH, Cimen-Bozkus C, Giles JR, Good Z, Goodman D, Jonsson VD, Lareau C, Marson A, Maurer DM, Munson PV, Stubbington M, Taylor S, Cutchin A| | PubMed
Improving prime editing with an endogenous small RNA-binding protein.
Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y, Simpson D, Zhao A, Li H, Yan W, Goudy L, Schmidt R, Solley SC, Gilbert LA, Chan MM, Bauer DE, Marson A, Parsons LR, Adamson B| | PubMed
CRISPR-Cas9 screen of E3 ubiquitin ligases identifies TRAF2 and UHRF1 as regulators of HIV latency in primary human T cells.
Rathore U, Haas P, Easwar Kumar V, Hiatt J, Haas KM, Bouhaddou M, Swaney DL, Stevenson E, Zuliani-Alvarez L, McGregor MJ, Turner-Groth A, Ochieng' Olwal C, Bediako Y, Braberg H, Soucheray M, Ott M, Eckhardt M, Hultquist JF, Marson A, Kaake RM, Krogan NJ| | PubMed
Ultra-high efficiency T cell reprogramming at multiple loci with SEED-Selection.
Chang CR, Vykunta VS, Goodman DB, Muldoon JJ, Nyberg WA, Liu C, Allain V, Rothrock A, Wang CH, Marson A, Shy BR, Eyquem J| | PubMed
The lncRNA Malat1 inhibits miR-15/16 to enhance cytotoxic T cell activation and memory cell formation.
Wheeler BD, Gagnon JD, Zhu WS, Muñoz-Sandoval P, Wong SK, Simeonov DS, Li Z, DeBarge R, Spitzer MH, Marson A, Ansel KM| | PubMed
Base-editing mutagenesis maps alleles to tune human T cell functions.
Schmidt R, Ward CC, Dajani R, Armour-Garb Z, Ota M, Allain V, Hernandez R, Layeghi M, Xing G, Goudy L, Dorovskyi D, Wang C, Chen YY, Ye CJ, Shy BR, Gilbert LA, Eyquem J, Pritchard JK, Dodgson SE, Marson A| | PubMed
Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein.
Bevacqua RJ, Zhao W, Merheb E, Kim SH, Marson A, Gloyn AL, Kim SK| | PubMed
PIM kinases regulate early human Th17 cell differentiation.
Buchacher T, Shetty A, Koskela SA, Smolander J, Kaukonen R, Sousa AGG, Junttila S, Laiho A, Rundquist O, Lönnberg T, Marson A, Rasool O, Elo LL, Lahesmaa R| | PubMed
Gene regulatory network inference from CRISPR perturbations in primary CD4+ T cells elucidates the genomic basis of immune disease.
Weinstock JS, Arce MM, Freimer JW, Ota M, Marson A, Battle A, Pritchard JK| | PubMed
HIC1 interacts with FOXP3 multi protein complex: Novel pleiotropic mechanisms to regulate human regulatory T cell differentiation and function.
Andrabi SBA, Batkulwar K, Bhosale SD, Moulder R, Khan MH, Buchacher T, Khan MM, Arnkil I, Rasool O, Marson A, Kalim UU, Lahesmaa R| | PubMed
Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein.
Bevacqua RJ, Zhao W, Merheb E, Kim SH, Marson A, Gloyn AL, Kim SK| | PubMed
Modular pooled discovery of synthetic knockin sequences to program durable cell therapies.
Blaeschke F, Chen YY, Apathy R, Daniel B, Chen AY, Chen PA, Sandor K, Zhang W, Li Z, Mowery CT, Yamamoto TN, Nyberg WA, To A, Yu R, Bueno R, Kim MC, Schmidt R, Goodman DB, Feuchtinger T, Eyquem J, Jimmie Ye C, Carnevale J, Satpathy AT, Shifrut E, Roth TL, Marson A| | PubMed
CRISPR screens decode cancer cell pathways that trigger γδ T cell detection.
Mamedov MR, Vedova S, Freimer JW, Sahu AD, Ramesh A, Arce MM, Meringa AD, Ota M, Chen PA, Hanspers K, Nguyen VQ, Takeshima KA, Rios AC, Pritchard JK, Kuball J, Sebestyen Z, Adams EJ, Marson A| | PubMed
The lncRNA Malat1 Inhibits miR-15/16 to Enhance Cytotoxic T Cell Activation and Memory Cell Formation.
Wheeler BD, Gagnon JD, Zhu WS, Muñoz-Sandoval P, Wong SK, Simeonov DR, Li Z, Debarge R, Spitzer MH, Marson A, Ansel KM| | PubMed
Non-coding sequence variation reveals fragility within interleukin 2 feedback circuitry and shapes autoimmune disease risk.
Simeonov DR, Park K, Cortez JT, Young A, Li Z, Nguyen V, Umhoefer J, Indart AC, Woo JM, Anderson MS, Tsang JS, Germain RN, Wong HS, Marson A| | PubMed
Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes.
Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, Allain V, Li Z, Marson A, Eyquem J, Wilson RC| | PubMed
Dynamic phosphatase-recruitment controls B-cell selection and oncogenic signaling.
Lee J, Robinson ME, Sun R, Kume K, Ma N, Cosgun KN, Chan LN, Leveille E, Geng H, Vykunta VS, Shy BR, Marson A, Katz S, Chen J, Paietta E, Meffre E, Vaidehi N, Müschen M| | PubMed
A single-amino acid substitution in the adaptor LAT accelerates TCR proofreading kinetics and alters T-cell selection, maintenance and function.
Lo WL, Kuhlmann M, Rizzuto G, Ekiz HA, Kolawole EM, Revelo MP, Andargachew R, Li Z, Tsai YL, Marson A, Evavold BD, Zehn D, Weiss A| | PubMed
Pooled screening of CAR T cells identifies diverse immune signaling domains for next-generation immunotherapies.
Goodman DB, Azimi CS, Kearns K, Talbot A, Garakani K, Garcia J, Patel N, Hwang B, Lee D, Park E, Vykunta VS, Shy BR, Ye CJ, Eyquem J, Marson A, Bluestone JA, Roybal KT| | PubMed
Advancing T cell-based cancer therapy with single-cell technologies.
Bucktrout SL, Banovich NE, Butterfield LH, Cimen-Bozkus C, Giles JR, Good Z, Goodman D, Jonsson VD, Lareau C, Marson A, Maurer DM, Munson PV, Stubbington M, Taylor S, Cutchin A| | PubMed
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails.
Shy BR, Vykunta VS, Ha A, Talbot A, Roth TL, Nguyen DN, Pfeifer WG, Chen YY, Blaeschke F, Shifrut E, Vedova S, Mamedov MR, Chung JJ, Li H, Yu R, Wu D, Wolf J, Martin TG, Castro CE, Ye L, Esensten JH, Eyquem J, Marson A| | PubMed
RASA2 ablation in T cells boosts antigen sensitivity and long-term function.
Carnevale J, Shifrut E, Kale N, Nyberg WA, Blaeschke F, Chen YY, Li Z, Bapat SP, Diolaiti ME, O'Leary P, Vedova S, Belk J, Daniel B, Roth TL, Bachl S, Anido AA, Prinzing B, Ibañez-Vega J, Lange S, Haydar D, Luetke-Eversloh M, Born-Bony M, Hegde B, Kogan S, Feuchtinger T, Okada H, Satpathy AT, Shannon K, Gottschalk S, Eyquem J, Krenciute G, Ashworth A, Marson A| | PubMed
A single-cell transcriptional gradient in human cutaneous memory T cells restricts Th17/Tc17 identity.
Cook CP, Taylor M, Liu Y, Schmidt R, Sedgewick A, Kim E, Hailer A, North JP, Harirchian P, Wang H, Kashem SW, Shou Y, McCalmont TC, Benz SC, Choi J, Purdom E, Marson A, Ramos SBV, Cheng JB, Cho RJ| | PubMed
Systematic discovery and perturbation of regulatory genes in human T cells reveals the architecture of immune networks.
Freimer JW, Shaked O, Naqvi S, Sinnott-Armstrong N, Kathiria A, Garrido CM, Chen AF, Cortez JT, Greenleaf WJ, Pritchard JK, Marson A| | PubMed
Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence.
Belk JA, Yao W, Ly N, Freitas KA, Chen YT, Shi Q, Valencia AM, Shifrut E, Kale N, Yost KE, Duffy CV, Daniel B, Hwee MA, Miao Z, Ashworth A, Mackall CL, Marson A, Carnevale J, Vardhana SA, Satpathy AT| | PubMed
Global post-translational modification profiling of HIV-1-infected cells reveals mechanisms of host cellular pathway remodeling.
Johnson JR, Crosby DC, Hultquist JF, Kurland AP, Adhikary P, Li D, Marlett J, Swann J, Hüttenhain R, Verschueren E, Johnson TL, Newton BW, Shales M, Simon VA, Beltrao P, Frankel AD, Marson A, Cox JS, Fregoso OI, Young JAT, Krogan NJ| | PubMed
A functional map of HIV-host interactions in primary human T cells.
Hiatt J, Hultquist JF, McGregor MJ, Bouhaddou M, Leenay RT, Simons LM, Young JM, Haas P, Roth TL, Tobin V, Wojcechowskyj JA, Woo JM, Rathore U, Cavero DA, Shifrut E, Nguyen TT, Haas KM, Malik HS, Doudna JA, May AP, Marson A, Krogan NJ| | PubMed
Obesity alters pathology and treatment response in inflammatory disease.
Bapat SP, Whitty C, Mowery CT, Liang Y, Yoo A, Jiang Z, Peters MC, Zhang LJ, Vogel I, Zhou C, Nguyen VQ, Li Z, Chang C, Zhu WS, Hastie AT, He H, Ren X, Qiu W, Gayer SG, Liu C, Choi EJ, Fassett M, Cohen JN, Sturgill JL, Crotty Alexander LE, Suh JM, Liddle C, Atkins AR, Yu RT, Downes M, Liu S, Nikolajczyk BS, Lee IK, Guttman-Yassky E, Ansel KM, Woodruff PG, Fahy JV, Sheppard D, Gallo RL, Ye CJ, Evans RM, Zheng Y, Marson A| | PubMed
Rusu I, Mennillo E, Bain JL, Li Z, Sun X, Ly KM, Rosli YY, Naser M, Wang Z, Advincula R, Achacoso P, Shao L, Razani B, Klein OD, Marson A, Turnbaugh JA, Turnbaugh PJ, Malynn BA, Ma A, Kattah MG| | PubMed
Hypoxia Is a Dominant Remodeler of the Effector T Cell Surface Proteome Relative to Activation and Regulatory T Cell Suppression.
Byrnes JR, Weeks AM, Shifrut E, Carnevale J, Kirkemo L, Ashworth A, Marson A, Wells JA| | PubMed
CRISPR-Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells.
Lin-Shiao E, Pfeifer WG, Shy BR, Saffari Doost M, Chen E, Vykunta VS, Hamilton JR, Stahl EC, Lopez DM, Sandoval Espinoza CR, Deyanov AE, Lew RJ, Poirer MG, Marson A, Castro CE, Doudna JA| | PubMed
CD97 promotes spleen dendritic cell homeostasis through the mechanosensing of red blood cells.
Liu D, Duan L, Rodda LB, Lu E, Xu Y, An J, Qiu L, Liu F, Looney MR, Yang Z, Allen CDC, Li Z, Marson A, Cyster JG| | PubMed
CRISPR activation and interference screens decode stimulation responses in primary human T cells.
Schmidt R, Steinhart Z, Layeghi M, Freimer JW, Bueno R, Nguyen VQ, Blaeschke F, Ye CJ, Marson A| | PubMed
ATF7ip Targets Transposable Elements for H3K9me3 Deposition to Modify CD8+ T Cell Effector and Memory Responses.
Sin JH, Kashyap S, Acenas D, Cortez JT, Lee J, Marson A, Matloubian M, Waterfield MR| | PubMed
Robust T cell activation requires an eIF3-driven burst in T cell receptor translation.
De Silva D, Ferguson L, Chin GH, Smith BE, Apathy RA, Roth TL, Blaeschke F, Kudla M, Marson A, Ingolia NT, Cate JH| | PubMed
PICS2: next-generation fine mapping via probabilistic identification of causal SNPs.
Taylor KE, Ansel KM, Marson A, Criswell LA, Farh KK| | PubMed
Using routinely recorded data in a UK RCT: a comparison to standard prospective data collection methods.
Powell GA, Bonnett LJ, Smith CT, Hughes DA, Williamson PR, Marson AG| | PubMed
Genome editing to define the function of risk loci and variants in rheumatic disease.
Baglaenko Y, Macfarlane D, Marson A, Nigrovic PA, Raychaudhuri S| | PubMed
Abcc1 and Ggt5 support lymphocyte guidance through export and catabolism of S-geranylgeranyl-l-glutathione.
Gallman AE, Wolfreys FD, Nguyen DN, Sandy M, Xu Y, An J, Li Z, Marson A, Lu E, Cyster JG| | PubMed
Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering.
Hamilton JR, Tsuchida CA, Nguyen DN, Shy BR, McGarrigle ER, Sandoval Espinoza CR, Carr D, Blaeschke F, Marson A, Doudna JA| | PubMed
Efficient generation of isogenic primary human myeloid cells using CRISPR-Cas9 ribonucleoproteins.
Hiatt J, Cavero DA, McGregor MJ, Zheng W, Budzik JM, Roth TL, Haas KM, Wu D, Rathore U, Meyer-Franke A, Bouzidi MS, Shifrut E, Lee Y, Kumar VE, Dang EV, Gordon DE, Wojcechowskyj JA, Hultquist JF, Fontaine KA, Pillai SK, Cox JS, Ernst JD, Krogan NJ, Marson A| | PubMed
XYZeq: Spatially resolved single-cell RNA sequencing reveals expression heterogeneity in the tumor microenvironment.
Lee Y, Bogdanoff D, Wang Y, Hartoularos GC, Woo JM, Mowery CT, Nisonoff HM, Lee DS, Sun Y, Lee J, Mehdizadeh S, Cantlon J, Shifrut E, Ngyuen DN, Roth TL, Song YS, Marson A, Chow ED, Ye CJ| | PubMed
The CD28-Transmembrane Domain Mediates Chimeric Antigen Receptor Heterodimerization With CD28.
Dysregulated RASGRP1 expression through RUNX1 mediated transcription promotes autoimmunity.
Baars MJD, Douma T, Simeonov DR, Myers DR, Kulhanek K, Banerjee S, Zwakenberg S, Baltissen MP, Amini M, de Roock S, van Wijk F, Vermeulen M, Marson A, Roose JP, Vercoulen Y| | PubMed
Thalamohippocampal atrophy in focal epilepsy of unknown cause at the time of diagnosis.
Leek NJ, Neason M, Kreilkamp BAK, de Bezenac C, Ziso B, Elkommos S, Das K, Marson AG, Keller SS| | PubMed
Author Correction: Multiplexed droplet single-cell RNA-sequencing using natural genetic variation.
Kang HM, Subramaniam M, Targ S, Nguyen M, Maliskova L, McCarthy E, Wan E, Wong S, Byrnes L, Lanata CM, Gate RE, Mostafavi S, Marson A, Zaitlen N, Criswell LA, Ye CJ| | PubMed
Functional CRISPR dissection of gene networks controlling human regulatory T cell identity.
Schumann K, Raju SS, Lauber M, Kolb S, Shifrut E, Cortez JT, Skartsis N, Nguyen VQ, Woo JM, Roth TL, Yu R, Nguyen MLT, Simeonov DR, Nguyen DN, Targ S, Gate RE, Tang Q, Bluestone JA, Spitzer MH, Ye CJ, Marson A| | PubMed
Layilin augments integrin activation to promote antitumor immunity.
Mahuron KM, Moreau JM, Glasgow JE, Boda DP, Pauli ML, Gouirand V, Panjabi L, Grewal R, Luber JM, Mathur AN, Feldman RM, Shifrut E, Mehta P, Lowe MM, Alvarado MD, Marson A, Singer M, Wells J, Jupp R, Daud AI, Rosenblum MD| | PubMed
NR4A nuclear receptors restrain B cell responses to antigen when second signals are absent or limiting.
Tan C, Hiwa R, Mueller JL, Vykunta V, Hibiya K, Noviski M, Huizar J, Brooks JF, Garcia J, Heyn C, Li Z, Marson A, Zikherman J| | PubMed
Evaluation of SARS-CoV-2 serology assays reveals a range of test performance.
Whitman JD, Hiatt J, Mowery CT, Shy BR, Yu R, Yamamoto TN, Rathore U, Goldgof GM, Whitty C, Woo JM, Gallman AE, Miller TE, Levine AG, Nguyen DN, Bapat SP, Balcerek J, Bylsma SA, Lyons AM, Li S, Wong AW, Gillis-Buck EM, Steinhart ZB, Lee Y, Apathy R, Lipke MJ, Smith JA, Zheng T, Boothby IC, Isaza E, Chan J, Acenas DD, Lee J, Macrae TA, Kyaw TS, Wu D, Ng DL, Gu W, York VA, Eskandarian HA, Callaway PC, Warrier L, Moreno ME, Levan J, Torres L, Farrington LA, Loudermilk RP, Koshal K, Zorn KC, Garcia-Beltran WF, Yang D, Astudillo MG, Bernstein BE, Gelfand JA, Ryan ET, Charles RC, Iafrate AJ, Lennerz JK, Miller S, Chiu CY, Stramer SL, Wilson MR, Manglik A, Ye CJ, Krogan NJ, Anderson MS, Cyster JG, Ernst JD, Wu AHB, Lynch KL, Bern C, Hsu PD, Marson A| | PubMed
Rewired signaling network in T cells expressing the chimeric antigen receptor (CAR).
Dong R, Libby KA, Blaeschke F, Fuchs W, Marson A, Vale RD, Su X| | PubMed
Test performance evaluation of SARS-CoV-2 serological assays.
Whitman JD, Hiatt J, Mowery CT, Shy BR, Yu R, Yamamoto TN, Rathore U, Goldgof GM, Whitty C, Woo JM, Gallman AE, Miller TE, Levine AG, Nguyen DN, Bapat SP, Balcerek J, Bylsma SA, Lyons AM, Li S, Wong AW, Gillis-Buck EM, Steinhart ZB, Lee Y, Apathy R, Lipke MJ, Smith JA, Zheng T, Boothby IC, Isaza E, Chan J, Acenas DD, Lee J, Macrae TA, Kyaw TS, Wu D, Ng DL, Gu W, York VA, Eskandarian HA, Callaway PC, Warrier L, Moreno ME, Levan J, Torres L, Farrington LA, Loudermilk R, Koshal K, Zorn KC, Garcia-Beltran WF, Yang D, Astudillo MG, Bernstein BE, Gelfand JA, Ryan ET, Charles RC, Iafrate AJ, Lennerz JK, Miller S, Chiu CY, Stramer SL, Wilson MR, Manglik A, Ye CJ, Krogan NJ, Anderson MS, Cyster JG, Ernst JD, Wu AHB, Lynch KL, Bern C, Hsu PD, Marson A| | PubMed
CRISPR screen in regulatory T cells reveals modulators of Foxp3.
Cortez JT, Montauti E, Shifrut E, Gatchalian J, Zhang Y, Shaked O, Xu Y, Roth TL, Simeonov DR, Zhang Y, Chen S, Li Z, Woo JM, Ho J, Vogel IA, Prator GY, Zhang B, Lee Y, Sun Z, Ifergan I, Van Gool F, Hargreaves DC, Bluestone JA, Marson A, Fang D| | PubMed
Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies.
Roth TL, Li PJ, Blaeschke F, Nies JF, Apathy R, Mowery C, Yu R, Nguyen MLT, Lee Y, Truong A, Hiatt J, Wu D, Nguyen DN, Goodman D, Bluestone JA, Ye CJ, Roybal K, Shifrut E, Marson A| | PubMed
Cyclophilin A Prevents HIV-1 Restriction in Lymphocytes by Blocking Human TRIM5α Binding to the Viral Core.
Selyutina A, Persaud M, Simons LM, Bulnes-Ramos A, Buffone C, Martinez-Lopez A, Scoca V, Di Nunzio F, Hiatt J, Marson A, Krogan NJ, Hultquist JF, Diaz-Griffero F| | PubMed
A Quantitative Genetic Interaction Map of HIV Infection.
Gordon DE, Watson A, Roguev A, Zheng S, Jang GM, Kane J, Xu J, Guo JZ, Stevenson E, Swaney DL, Franks-Skiba K, Verschueren E, Shales M, Crosby DC, Frankel AD, Marson A, Marazzi I, Cagney G, Krogan NJ| | PubMed
Regulatory T cells use arginase 2 to enhance their metabolic fitness in tissues.
Lowe MM, Boothby I, Clancy S, Ahn RS, Liao W, Nguyen DN, Schumann K, Marson A, Mahuron KM, Kingsbury GA, Liu Z, Munoz Sandoval P, Rodriguez RS, Pauli ML, Taravati K, Arron ST, Neuhaus IM, Harris HW, Kim EA, Shin US, Krummel MF, Daud A, Scharschmidt TC, Rosenblum MD| | PubMed
A Mutation in the Transcription Factor Foxp3 Drives T Helper 2 Effector Function in Regulatory T Cells.
Van Gool F, Nguyen MLT, Mumbach MR, Satpathy AT, Rosenthal WL, Giacometti S, Le DT, Liu W, Brusko TM, Anderson MS, Rudensky AY, Marson A, Chang HY, Bluestone JA| | PubMed
Thymic regulatory T cells arise via two distinct developmental programs.
Owen DL, Mahmud SA, Sjaastad LE, Williams JB, Spanier JA, Simeonov DR, Ruscher R, Huang W, Proekt I, Miller CN, Hekim C, Jeschke JC, Aggarwal P, Broeckel U, LaRue RS, Henzler CM, Alegre ML, Anderson MS, August A, Marson A, Zheng Y, Williams CB, Farrar MA| | PubMed
CRISPR-Cas9 genome engineering of primary CD4+ T cells for the interrogation of HIV-host factor interactions.
CRL4AMBRA1 targets Elongin C for ubiquitination and degradation to modulate CRL5 signaling.
Chen SH, Jang GM, Hüttenhain R, Gordon DE, Du D, Newton BW, Johnson JR, Hiatt J, Hultquist JF, Johnson TL, Liu YL, Burton LA, Ye J, Reichermeier KM, Stroud RM, Marson A, Debnath J, Gross JD, Krogan NJ| | PubMed
Reprogramming human T cell function and specificity with non-viral genome targeting.
Roth TL, Puig-Saus C, Yu R, Shifrut E, Carnevale J, Li PJ, Hiatt J, Saco J, Krystofinski P, Li H, Tobin V, Nguyen DN, Lee MR, Putnam AL, Ferris AL, Chen JW, Schickel JN, Pellerin L, Carmody D, Alkorta-Aranburu G, Del Gaudio D, Matsumoto H, Morell M, Mao Y, Cho M, Quadros RM, Gurumurthy CB, Smith B, Haugwitz M, Hughes SH, Weissman JS, Schumann K, Esensten JH, May AP, Ashworth A, Kupfer GM, Greeley SAW, Bacchetta R, Meffre E, Roncarolo MG, Romberg N, Herold KC, Ribas A, Leonetti MD, Marson A| | PubMed
Revisiting IL-2: Biology and therapeutic prospects.
Abbas AK, Trotta E, R Simeonov D, Marson A, Bluestone JA| | PubMed
Author Correction: Discovery of stimulation-responsive immune enhancers with CRISPR activation.
Simeonov DR, Gowen BG, Boontanrart M, Roth TL, Gagnon JD, Mumbach MR, Satpathy AT, Lee Y, Bray NL, Chan AY, Lituiev DS, Nguyen ML, Gate RE, Subramaniam M, Li Z, Woo JM, Mitros T, Ray GJ, Curie GL, Naddaf N, Chu JS, Ma H, Boyer E, Van Gool F, Huang H, Liu R, Tobin VR, Schumann K, Daly MJ, Farh KK, Ansel KM, Ye CJ, Greenleaf WJ, Anderson MS, Bluestone JA, Chang HY, Corn JE, Marson A| | PubMed
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms.
Farboud B, Jarvis E, Roth TL, Shin J, Corn JE, Marson A, Meyer BJ, Patel NH, Hochstrasser ML| | PubMed
Light-activated cell identification and sorting (LACIS) for selection of edited clones on a nanofluidic device.
Mocciaro A, Roth TL, Bennett HM, Soumillon M, Shah A, Hiatt J, Chapman K, Marson A, Lavieu G| | PubMed
Genetic engineering in primary human B cells with CRISPR-Cas9 ribonucleoproteins.
Wu CM, Roth TL, Baglaenko Y, Ferri DM, Brauer P, Zuniga-Pflucker JC, Rosbe KW, Wither JE, Marson A, Allen CDC| | PubMed
Multiplexed droplet single-cell RNA-sequencing using natural genetic variation.
Kang HM, Subramaniam M, Targ S, Nguyen M, Maliskova L, McCarthy E, Wan E, Wong S, Byrnes L, Lanata CM, Gate RE, Mostafavi S, Marson A, Zaitlen N, Criswell LA, Ye CJ| | PubMed
Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements.
Mumbach MR, Satpathy AT, Boyle EA, Dai C, Gowen BG, Cho SW, Nguyen ML, Rubin AJ, Granja JM, Kazane KR, Wei Y, Nguyen T, Greenside PG, Corces MR, Tycko J, Simeonov DR, Suliman N, Li R, Xu J, Flynn RA, Kundaje A, Khavari PA, Marson A, Corn JE, Quertermous T, Greenleaf WJ, Chang HY| | PubMed
Discovery of stimulation-responsive immune enhancers with CRISPR activation.
Simeonov DR, Gowen BG, Boontanrart M, Roth TL, Gagnon JD, Mumbach MR, Satpathy AT, Lee Y, Bray NL, Chan AY, Lituiev DS, Nguyen ML, Gate RE, Subramaniam M, Li Z, Woo JM, Mitros T, Ray GJ, Curie GL, Naddaf N, Chu JS, Ma H, Boyer E, Van Gool F, Huang H, Liu R, Tobin VR, Schumann K, Daly MJ, Farh KK, Ansel KM, Ye CJ, Greenleaf WJ, Anderson MS, Bluestone JA, Chang HY, Corn JE, Marson A| | PubMed
Using routinely recorded data in the UK to assess outcomes in a randomised controlled trial: The Trials of Access.
Epilepsy and Pregnancy: For healthy pregnancies and happy outcomes. Suggestions for service improvements from the Multispecialty UK Epilepsy Mortality Group.
Van Dyken SJ, Nussbaum JC, Lee J, Molofsky AB, Liang HE, Pollack JL, Gate RE, Haliburton GE, Ye CJ, Marson A, Erle DJ, Locksley RM| | PubMed
Generation of knock-in primary human T cells using Cas9 ribonucleoproteins.
Schumann K, Lin S, Boyer E, Simeonov DR, Subramaniam M, Gate RE, Haliburton GE, Ye CJ, Bluestone JA, Doudna JA, Marson A| | PubMed
'Seizure First Aid Training' for people with epilepsy who attend emergency departments, and their family and friends: study protocol for intervention development and a pilot randomised controlled trial.
Noble AJ, Marson AG, Tudur-Smith C, Morgan M, Hughes DA, Goodacre S, Ridsdale L| | PubMed
Kofler DM, Marson A, Dominguez-Villar M, Xiao S, Kuchroo VK, Hafler DA| | PubMed
Small-molecule RORγt antagonists inhibit T helper 17 cell transcriptional network by divergent mechanisms.
Xiao S, Yosef N, Yang J, Wang Y, Zhou L, Zhu C, Wu C, Baloglu E, Schmidt D, Ramesh R, Lobera M, Sundrud MS, Tsai PY, Xiang Z, Wang J, Xu Y, Lin X, Kretschmer K, Rahl PB, Young RA, Zhong Z, Hafler DA, Regev A, Ghosh S, Marson A, Kuchroo VK| | PubMed
A comprehensive functional and clinical analysis of ABCC2 and its impact on treatment response to carbamazepine.
Rädisch S, Dickens D, Lang T, Bonnett L, Arlanov R, Johnson MR, Schwab M, Marson AG, Pirmohamed M| | PubMed
Successful medical management of a Nocardia farcinica multiloculated pontine abscess.
Transcriptional role of cyclin D1 in development revealed by a genetic-proteomic screen.
Bienvenu F, Jirawatnotai S, Elias JE, Meyer CA, Mizeracka K, Marson A, Frampton GM, Cole MF, Odom DT, Odajima J, Geng Y, Zagozdzon A, Jecrois M, Young RA, Liu XS, Cepko CL, Gygi SP, Sicinski P| | PubMed
Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells.
Marson A, Levine SS, Cole MF, Frampton GM, Brambrink T, Johnstone S, Guenther MG, Johnston WK, Wernig M, Newman J, Calabrese JM, Dennis LM, Volkert TL, Gupta S, Love J, Hannett N, Sharp PA, Bartel DP, Jaenisch R, Young RA| | PubMed
Wnt signaling promotes reprogramming of somatic cells to pluripotency.
Marson A, Foreman R, Chevalier B, Bilodeau S, Kahn M, Young RA, Jaenisch R| | PubMed
Foxp3 occupancy and regulation of key target genes during T-cell stimulation.
Marson A, Kretschmer K, Frampton GM, Jacobsen ES, Polansky JK, MacIsaac KD, Levine SS, Fraenkel E, von Boehmer H, Young RA| | PubMed
PML bodies control the nuclear dynamics and function of the CHFR mitotic checkpoint protein.
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